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Journal of Chromatography B
Copyright © 2004 Elsevier B.V. All rights reserved

Volume 786, Issues 1-2, Pages 1-363 (25 March 2003)
European Contest for Young Scientists on Protein Purification: From Gene to Functional Protein

Paris, France, 10 - 11 June 2002
Edited by Michael Kaleja
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1.
Preface EDITORIAL
Pages 1-2

SummaryPlus | Full Text + Links | PDF (43 K)
2.
Purification of the proline-rich homeodomain protein ARTICLE
Pages 3-6
Amy J. Butcher, Kevin Gaston and Padma-Sheela Jayaraman
SummaryPlus | Full Text + Links | PDF (254 K)
3.
Purification of human alpha-Image-fucosidase precursor expressed in Escherichia coli as a glutathione S-transferase fusion protein ARTICLE
Pages 7-15
Alejandro de Carlos, Dolores Montenegro, Ana Alonso-Rodríguez, María Páez de la Cadena, Francisco Javier Rodríguez-Berrocal and Vicenta Soledad Martínez-Zorzano
SummaryPlus | Full Text + Links | PDF (420 K)
4.
Mutation of surface-exposed histidine residues of recombinant human granulocyte-colony stimulating factor (Cys17Ser) impacts on interaction with chelated metal ions and refolding in aqueous two-phase systems ARTICLE
Pages 17-32
Mindaugas Zaveckas, Virginijus LukImagea, Gintautas Imagevirblis, Valerija ChmieliauskaitImage, Vladas Bumelis and Henrikas Pesliakas
SummaryPlus | Full Text + Links | PDF (613 K)
5.
Two-step purification of the outer membrane transporter and activator protein ShlB from Escherichia coli using internally His6-tagged constructs ARTICLE
Pages 33-37
S. R. Sauter, S. Diekmann and V. Braun
SummaryPlus | Full Text + Links | PDF (229 K)
6.
Purification of recombinantly expressed human cluster determinant 4 cytoplasmic domain ARTICLE
Pages 39-44
Andrea Preusser, Gesa Jonas and Dieter Willbold
SummaryPlus | Full Text + Links | PDF (174 K)
7.
Purification of recombinant BtpA and Ycf3, proteins involved in membrane protein biogenesis in Synechocystis PCC 6803 ARTICLE
Pages 45-59
Tatjana M. E. Schwabe, Kirsten Gloddek, Daniela Schluesener and Jochen Kruip
SummaryPlus | Full Text + Links | PDF (1057 K)
8.
Expression and purification of Plasmodium falciparum MSP-142: A malaria vaccine candidate ARTICLE
Pages 61-72
Christian Epp, Christian W. Kauth, Hermann Bujard and Rolf Lutz
SummaryPlus | Full Text + Links | PDF (655 K)
9.
Cloning, purification and biochemical characterization of dipetarudin, a new chimeric thrombin inhibitor ARTICLE
Pages 73-80
M. López, K. Mende, T. Steinmetzer and G. Nowak
SummaryPlus | Full Text + Links | PDF (356 K)
10.
Identification and characterisation of Schizosaccharomyces pombe cyclophilin 3, a cyclosporin A insensitive orthologue of human USA-CyP ARTICLE
Pages 81-91
Trevor J. Pemberton, Stuart L. Rulten and John E. Kay
SummaryPlus | Full Text + Links | PDF (900 K)
11.
Novel approach to obtain biologically active recombinant heterodimeric proteins in Escherichia coli ARTICLE
Pages 93-107
Corrine Austin
SummaryPlus | Full Text + Links | PDF (663 K)
12.
Recombinant expression and purification of Ssa1p (Hsp70) from Saccharomyces cerevisiae using Pichia pastoris ARTICLE
Pages 109-115
Harald Wegele, Martin Haslbeck and Johannes Buchner
SummaryPlus | Full Text + Links | PDF (323 K)
13.
Cloning, expression and purification of three Chaperonin 60 homologues ARTICLE
Pages 117-125
Maria Maguire, Anthony R. M. Coates and Brian Henderson
SummaryPlus | Full Text + Links | PDF (196 K)
14.
GroE-dependent expression and purification of pig heart mitochondrial citrate synthase in Escherichia coli ARTICLE
Pages 127-136
Martin Haslbeck, Ioana Schuster and Holger Grallert
SummaryPlus | Full Text + Links | PDF (925 K)
15.
In vitro cell free synthesis of human manganese superoxide dismutase with the RTS 500 system ARTICLE
Pages 137-142
Michael Kleines, Martin Nellessen, Lars Schaade and Klaus Ritter
SummaryPlus | Full Text + Links | PDF (416 K)
16.
Simplified procedure to recover recombinant antigenized secretory IgA to be used as a vaccine vector ARTICLE
Pages 143-151
Laurent I. Favre, François Spertini and Blaise Corthésy
SummaryPlus | Full Text + Links | PDF (584 K)
17.
Cloning, expression and two-step purification of recombinant His-tag enhanced green fluorescent protein over-expressed in Escherichia coli ARTICLE
Pages 153-159
W. Dieryck, A. M. Noubhani, D. Coulon and X. Santarelli
SummaryPlus | Full Text + Links | PDF (356 K)
18.
Optimizing expression and purification from cell culture medium of trispecific recombinant antibody derivatives ARTICLE
Pages 161-176
An Willems, Jannick Leoen, Steve Schoonooghe, Johan Grooten and Nico Mertens
SummaryPlus | Full Text + Links | PDF (1335 K)
19.
High-level expression and rapid purification of rare-codon genes from hyperthermophilic archaea by the GST gene fusion system ARTICLE
Pages 177-185
Xiaoqiu Wu and Udo Oppermann
SummaryPlus | Full Text + Links | PDF (316 K)
20.
Optimisation of expression and purification of the recombinant Yol066 (Rib2) protein from Saccharomyces cerevisiae ARTICLE
Pages 187-195
A. Urban, I. Ansmant and Y. Motorin
SummaryPlus | Full Text + Links | PDF (538 K)
21.
Overexpression and purification of the three components of the Enterobacter aerogenes AcrA–AcrB–TolC multidrug efflux pump ARTICLE
Pages 197-205
Muriel Masi, Jean-Marie Pagès and Elizabeth Pradel
SummaryPlus | Full Text + Links | PDF (366 K)
22.
Production of recombinant thermostable proteins expressed in Escherichia coli: completion of protein synthesis is the bottleneck ARTICLE
Pages 207-214
Hans Peter Sørensen, Hans Uffe Sperling-Petersen and Kim Kusk Mortensen
SummaryPlus | Full Text + Links | PDF (404 K)
23.
Heterologous expression and purification of native and mutated low molecular mass glutenin subunits from durum wheat ARTICLE
Pages 215-220
C. Patacchini, S. Masci, R. D’Ovidio and D. Lafiandra
SummaryPlus | Full Text + Links | PDF (282 K)
24.
Cloning and expression of fatty acids biosynthesis key enzymes from sunflower (Helianthus annuus L.) in Escherichia coli ARTICLE
Pages 221-228
María Josefa Serrano-Vega, Mónica Venegas-Calerón, Rafael Garcés and Enrique Martínez-Force
SummaryPlus | Full Text + Links | PDF (337 K)
25.
Optimisation of expression and immobilized metal ion affinity chromatographic purification of recombinant (His)6-tagged cytochrome P450 hydroperoxide lyase in Escherichia coli ARTICLE
Pages 229-236
Jérôme Delcarte, Marie-Laure Fauconnier, Philippe Jacques, Kenji Matsui, Philippe Thonart and Michel Marlier
SummaryPlus | Full Text + Links | PDF (339 K)
26.
Continuous processing of fusion protein expressed as an Escherichia coli inclusion body ARTICLE
Pages 237-246
Giacomo Morreale, Heikki Lanckriet, Jemma C. Miller and Anton P. J. Middelberg
SummaryPlus | Full Text + Links | PDF (601 K)
27.
A novel strategy for the purification of recombinantly expressed unstructured protein domains ARTICLE
Pages 247-254
Christoph Kalthoff
SummaryPlus | Full Text + Links | PDF (422 K)
28.
Taenia solium cDNA sequence encoding a putative immunodiagnostic antigen for human cysticercosis ARTICLE
Pages 255-269
E. Montero, L. M. González, L. J. S. Harrison, R. M. E. Parkhouse and T. Gárate
SummaryPlus | Full Text + Links | PDF (845 K)
29.
Large scale protein production of the extracellular domain of the transforming growth factor-small beta, Greek type II receptor using the Pichia pastoris expression system ARTICLE
Pages 271-277
Alwin Scharstuhl, Harrie Glansbeek, Elly L. Vitters, Peter M. van der Kraan and Wim B. van den Berg
SummaryPlus | Full Text + Links | PDF (226 K)
30.
Isolation of Qsmall beta, Greek polymerase complexes containing mutant species of elongation factor Tu ARTICLE
Pages 279-286
Sander G. J. Mathu, Charlotte R. Knudsen, Jan van Duin and Barend Kraal
SummaryPlus | Full Text + Links | PDF (603 K)
31.
Expression and purification of human cardiac troponin subunits and their functional incorporation into isolated cardiac mouse myofibrils ARTICLE
Pages 287-296
Martina Krüger, Gabriele Pfitzer and Robert Stehle
SummaryPlus | Full Text + Links | PDF (290 K)
32.
Production of a soluble small gamma, Greeksmall delta, Greek T-cell receptor to identify ligands for the murine intestinal intraepithelial small gamma, Greeksmall delta, Greek T cell population ARTICLE
Pages 297-304
Carrie R. Steele, Kier C. Van Remoortere and Adrian C. Hayday
SummaryPlus | Full Text + Links | PDF (525 K)
33.
On-column refolding of an insoluble histidine tag recombinant exopolyphosphatase from Trypanosoma brucei overexpressed in Escherichia coli ARTICLE
Pages 305-309
G. Lemercier, N. Bakalara and X. Santarelli
SummaryPlus | Full Text + Links | PDF (140 K)
34.
Identification of a tubulin binding motif on the P2X2 receptor ARTICLE
Pages 311-318
Sandra Gendreau, Jörg Schirmer and Günther Schmalzing
SummaryPlus | Full Text + Links | PDF (389 K)
35.
GST–Fbe can recognize small beta, Greek-chains of fibrin(ogen) on explanted materials ARTICLE
Pages 319-325
Lei Pei, Ingegerd Löfving Arvholm, Lena Lonnies and Jan-Ingmar Flock
SummaryPlus | Full Text + Links | PDF (613 K)
36.
Production, purification and characterisation of genetically derived scFv and bifunctional antibody fragments capable of detecting illicit drug residues ARTICLE
Pages 327-342
Joanne Brennan, Paul Dillon and Richard O’Kennedy
SummaryPlus | Full Text + Links | PDF (1167 K)
37.
Semi automated production of a set of different recombinant GST-Streptag fusion proteins ARTICLE
Pages 343-355
Petra Sebastian, Jelena Wallwitz and Stefan Schmidt
SummaryPlus | Full Text + Links | PDF (1482 K)
38.
Author Index to Vol. 786 MISCELLANEOUS
Pages 357-359

PDF (56 K)
39.
Compound Index to Vol. 786 MISCELLANEOUS
Pages 361-363

PDF (51 K)
40.
Editorial Board MISCELLANEOUS
Page iii

PDF (35 K)

Issue List
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